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Literacy assessment is essential for effective literacy instruction and training. However, traditional paper-based literacy assessments are typically decontextualized and may cause stress and anxiety for test takers. In contrast, serious games and game environments allow for the assessment of literacy in more authentic and engaging ways, which has some potential to increase the assessment’s validity and reliability. The primary objective of this study is to examine the feasibility of a novel approach for stealthily assessing literacy skills using games in an intelligent tutoring system (ITS) designed for reading comprehension strategy training. We investigated the degree to which learners’ game performance and enjoyment predicted their scores on standardized reading tests. Amazon Mechanical Turk participants (n = 211) played three games in iSTART and self-reported their level of game enjoyment after each game. Participants also completed the Gates–MacGinitie Reading Test (GMRT), which includes vocabulary knowledge and reading comprehension measures. The results indicated that participants’ performance in each game as well as the combined performance across all three games predicted their literacy skills. However, the relations between game enjoyment and literacy skills varied across games. These findings suggest the potential of leveraging serious games to assess students’ literacy skills and improve the adaptivity of game-based learning environments. 

Genomic imprinting is an epigenetic phenomenon in which differential allele expression occurs in a parent-of-origin-dependent manner. Imprinting in plants is tightly linked to transposable elements (TEs), and it has been hypothesized that genomic imprinting may be a consequence of demethylation of TEs. Here, we performed high-throughput sequencing of ribonucleic acids from four maize (Zea mays) endosperms that segregated newly silenced Mutator (Mu) transposons and identified 110 paternally expressed imprinted genes (PEGs) and 139 maternally expressed imprinted genes (MEGs). Additionally, two potentially novel paternally suppressed MEGs are associated with de novo Mu insertions. In addition, we find evidence for parent-of-origin effects on expression of 407 conserved noncoding sequences (CNSs) in maize endosperm. The imprinted CNSs are largely localized within genic regions and near genes, but the imprinting status of the CNSs are largely independent of their associated genes. Both imprinted CNSs and PEGs have been subject to relaxed selection. However, our data suggest that although MEGs were already subject to a higher mutation rate prior to their being imprinted, imprinting may be the cause of the relaxed selection of PEGs. In addition, although DNA methylation is lower in the maternal alleles of both the maternally and paternally expressed CNSs (mat and pat CNSs), the difference between the two alleles in H3K27me3 levels was only observed in pat CNSs. Together, our findings point to the importance of both transposons and CNSs in genomic imprinting in maize.

 

ABSTRACT Here, we present the complete chloroplast genome sequence of Toxicodendron diversilobum , western poison oak, from Pacific Grove, California. The genome is 159,543 bp in length, contains 133 genes, and has a high level of gene synteny to other species of Toxicodendron . 

Cellulose-based materials have gained increasing attention for the development of low cost, eco-friendly technologies, and more recently, as functional materials in triboelectric nanogenerators (TENGs). However, the low output performance of cellulose-based TENGs severely restricts their versatility and employment in emerging smart building and smart city applications. Here, we report a high output performance of a commercial cellulosic material-based energy harvesting floor (CEHF). Benefiting from the significant difference in the triboelectric properties between weighing and nitrocellulose papers, high surface roughness achieved by a newly developed mechanical exfoliation method, and large overall contact area via a multilayered device structure, the CEHF (25 cm × 15 cm × 1.2 cm) exhibits excellent output performance with a maximum output voltage, current, and power peak values of 360 V, 250 μA, and 5 mW, respectively. It can be directly installed or integrated with regular flooring products to effectively convert human body movements into electricity and shows good durability and stability. Moreover, a wireless transmission sensing system that can produce a 1:1 footstep-to-signal (transmitted and received) ratio is instantaneously powered by a TENG based entirely on cellulosic materials for the first time. This work provides a feasible and effective way to utilize commercial cellulosic materials to construct self-powered wireless transmission systems for real-time sensing applications. 

The shared nature of the wireless medium induces contention between data transport and backward signaling, such as acknowledgement. The current way of TCP acknowledgment induces control overhead which is counter-productive for TCP performance especially in wireless local area network (WLAN) scenarios.In this paper, we present a new acknowledgement called TACK ("Tame ACK"), as well as its TCP implementation TCP-TACK. TCP-TACK works on top of commodity WLAN, delivering high wireless transport goodput with minimal control overhead in the form of ACKs, without any hardware modification. To minimize ACK frequency, TACK abandons the legacy received-packet-driven ACK. Instead, it balances byte-counting ACK and periodic ACK so as to achieve a controlled ACK frequency. Evaluation results show that TCP-TACK achieves significant advantages over legacy TCP in WLAN scenarios due to less contention between data packets and ACKs. Specifically, TCP-TACK reduces over 90% of ACKs and also obtains an improvement of ~ 28% on good-put. We further find it performs equally well as high-speed TCP variants in wide area network (WAN) scenarios, this is attributed to the advancements of the TACK-based protocol design in loss recovery, round-trip timing, and send rate control. 

The shared nature of the wireless medium induces contention between data transport and backward signaling, such as acknowledgment. The current way of TCP acknowledgment induces control overhead which is counter-productive for TCP performance especially in wireless local area network (WLAN) scenarios. In this paper, we present a new acknowledgment called TACK (“Tame ACK”), as well as its TCP implementation TCP-TACK. TACK seeks to minimize ACK frequency, which is exactly what is required by transport. TCP-TACK works on top of commodity WLAN, delivering high wireless transport goodput with minimal control overhead in the form of ACKs, without any hardware modification. Evaluation results show that TCP-TACK achieves significant advantages over legacy TCP in WLAN scenarios due to less contention between data packets and ACKs. Specifically, TCP-TACK reduces over 90% of ACKs and also obtains an improvement of up to 28% on goodput. A TACK-based protocol is a good replacement of the legacy TCP to compensate for scenarios where the acknowledgment overhead is non-negligible.